Yes.

Windows

At the "Execute file" dialog on the Start menu, input "mmsys.cpl" and press "OK". An audio settings window opens. At the [Sound Playback] audio tab, select "Pioneer DJM-900nexus". Input the signal to this unit's channel 1. Signals cannot be input to other channels.

*This cannot be used at the same time as an ASIO driver.

Mac OS

At the [Output] tab at [System Preferences] -> [Sound], select "Audio output device", then select "DJM-900nexus" from the sound devices. The output channels to this unit can be changed by selecting "DJM-900nexus" at [Audio MIDI Settings] under [Applications] -> [Utilities] then using the [Configure Speakers ...] dialog.