Yes they can.

For Windows 8, Windows 7 and Windows Vista

1. At the [Run] dialog box in the start menu, input “mmsys.cpl”, then press OK.

2. The sound window opens. At the Sound tab, select [PIONEER DJM-750].

For Windows XP

1. At the [Run] dialog box in the start menu, input “mmsys.cpl”, then press OK.

2. The audio settings window opens. At [Audio Playback] in the Sound tab, select [PIONEER DJM-750].

In both of the above cases, the signals are input to this unit’s channel 1. They cannot be input to other channels.

*This cannot be used simultaneously with the ASIO driver.

For Mac OS

At the [Output] tab at [System Preferences] – [Sound], select [Sound Output Device], then select [PIONEER DJM-750] from the sound devices.

The channel of output to the DJM-750 can be changed by selecting [PIONEER DJM-750] at [Applications] – [Utilities] – [Audio MIDI Settings] and using the [Configure Speakers...] dialog box.